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KMID : 1034820220180030321
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Molecular & Cellular Toxicology
2022 Volume.18 No. 3 p.321 ~ p.327
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Esculentoside A exerts anti-oxidative stress and anti-apoptotic effects in rat experimental membranous nephropathy by regulating MAPK pathway
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Wu Jin
Lei Genping
Wang Ting
Dong Sheng
Zhan Xiaolin
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Abstract
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Background: Membranous nephropathy is characterized by deposition of immune complex. The initial inflammatory responses induce cell apoptosis and oxidative injury, thus contributing to the pathogenesis of chronic kidney disease. Esculentoside A exerts anti-inflammatory, anti-apoptotic and anti-oxidant abilities, and protects against acute kidney injury.
Objective: The renoprotective effect of Esculentoside A on a rat model with membranous nephropathy was investigated in this study.
Results: Injection of cationic bovine serum albumin promoted the level of 24 h proteinuria, and induced histopathological damage, including glomerular atrophy and thick glomerular basement membrane in the kidney tissues. Intraperitoneal injection with Esculentoside A reduced the level of 24 h proteinuria, and attenuated the pathological damages. Esculentoside A attenuated cationic bovine serum albumin-induced reduction of Bcl-2 protein expression, enhancements in the protein expressions of Bax and cleaved caspase-3. Besides, the enhanced levels of MDA (malondialdehyde) and NO (nitric oxide), and reduced levels of SOD (superoxide dismutase) and GSH (glutathione), in rats with membranous nephropathy were restored by Esculentoside A injection. Moreover, Esculentoside A counteracted with the promotive effects of cationic bovine serum albumin on the protein expressions of phosphorylated JNK (p-JNK), p-ERK1/2 and p-p38.
Conclusion: The renoprotective effects of Esculentoside A on a rat model with membranous nephropathy was mediated by its anti-oxidant and anti-apoptotic effects through inactivation of MAPKs pathway.
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KEYWORD
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Esculentoside A, Oxidative stress, Apoptosis, Membranous nephropathy, MAPK, Cationic bovine serum albumin
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