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KMID : 1034820220180040549
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Molecular & Cellular Toxicology
2022 Volume.18 No. 4 p.549 ~ p.556
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Hyaluronic acid suppresses the effect of di-(2-ethylhexyl) phthalate in HaCaT keratinocytes
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Kim Jin-Hee
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Abstract
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Backgrounds: High molecular weight (HMW) hyaluronic acid (HA) is a major component of the extracellular matrix and functions as an anti-inflammatory and anti-permeability agent. Di-(2-ethylhexyl) phthalate (DEHP) is a widely used plasticizer in polymers and it is a common endocrine-disrupting compound that is found in the environment. In this study, we aimed to evaluate the effect of HMW-HA on skin disorders caused by DEHP.
Objectives: We determined the effect of HMW-HA on various cellular processes and pathways in HaCaT keratinocytes treated with DEHP.
Results: The results demonstrated that DEHP-induced cell proliferation significantly increased after HMW-HA treatment in HaCaT keratinocytes. Levels of matrix metalloproteinase (MMP)-2, MMP-9, phospho-nuclear factor-kappa B, cyclooxygenase-2, cleaved-caspase-3, and poly-adenosine diphosphate-ribose polymerase were significantly lower in cells exposed to HMW-HA than in DEHP-treated cells. In addition, DEHP-associated phosphorylation of protein kinase B, extracellular signal-regulated kinase 1/2, and p38 was lower in HMW-HA-treated cells than in DEHP-treated cells.
Conclusion: Therefore, our findings provide evidence that HMW-HA treatment involves the anti-inflammatory cell pathways that prevent or mitigate skin disorders caused by exposure to DEHP.
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KEYWORD
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High molecular weight hyaluronic acid, Di-(2-ethylhexyl) phthalate, Skin disorders, HaCaT keratinocytes
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