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KMID : 1034820230190010033
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Molecular & Cellular Toxicology
2023 Volume.19 No. 1 p.33 ~ p.39
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LGR4 protects PC12 against OGD/R-induced oxidative stress and apoptosis through activation of AKT/GSK3β
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Jing Pei
Liqin Luan
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Abstract
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Background : Leucine-rich repeat-containing G protein-coupled receptor 4 (LGR4) suppresses hypoxia/re-oxygenation-induced injury in hepatocytes, and protects against hepatic and myocardial ischemia/reperfusion (I/R)-induced injury. The role of LGR4 on cerebral I/R injury was investigated in this study.
Objective : Providing potential therapeutic target of cerebral I/R injury.
Results : Expression of LGR4 was reduced in OGD/R-induced PC12. Over-expression of LGR4 attenuated OGD/R-induced decrease of cell viability and increase of apoptosis. Increased levels of LDH, ROS, MDA, and MPO, as well as decreased SOD, in OGD/R-induced PC12, were restored by LGR4 over-expression. Over-expression of LGR4 counteracted with the suppressive effects of OGD/R on protein expression of AKT and GSK3β phosphorylation in PC12.
Conclusion : The protective effect of LGR4 against OGD/R-induced oxidative stress and apoptosis in PC12 was mediated by activation of AKT/GSK3β pathway.
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KEYWORD
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LGR4, Apoptosis, Oxidative stress, Oxygen?glucose deprivation/re-oxygenation, PC12, AKT/GSK3β
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