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KMID : 1034820230190010053
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Molecular & Cellular Toxicology
2023 Volume.19 No. 1 p.53 ~ p.62
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LncRNA SNHG12/miR-494-3p/CBX3 axis in diffuse large B-cell lymphoma
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Cheng Si
Wanyong Zhang
Qi Han
Bisheng Zhu
Chengzhi Zhan
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Abstract
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Background : Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoid malignancy in adults. Current studies have implicated that long non-coding RNAs (lncRNAs) regulate the development of DLBCL; nevertheless, the control method is dim. Here, we reconnoitered the characters of lncRNA small nucleolar RNA host gene 12 (lncRNA SNHG12) in DLBCL.
Objective : The pathological morphology of tumor tissues and normal tissues was perceived by hematoxylin?eosin (HE) staining. The levels of SNHG12, microRNA-494-3p (miR-494-3p), and chromobox 3 (CBX3) were detected by quantitative real-time PCR and western blot. Whereafter, MTT assay and EdU assay were enforced to validate cell proliferation. Flow cytometry assay was implemented to assess the cell cycle and cell apoptosis, respectively. Additionally, the interface between miR-494-3p with SNHG12 or CBX3 was identified by dual-luciferase reporter assay.
Results : SNHG12 and CBX3 were enhanced, but miR-494-3p was diminished in DLBCL. Knockdown of SNHG12 repressed cell proliferation and cell cycle, although heightened cell apoptosis in DLBCL cells. SNHG12 sponged miR-494-3p to adjust the CBX3. Additionally, miR-494-3p restrained DLBCL cells development by targeting CBX3.
Conclusion : SNHG12 contributed to DLBCL development by regulating miR-494-3p/CBX3. The outcomes demonstrated that SNHG12 provided a potential train of thought for DLBCL theory of targeted therapy.
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KEYWORD
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Diffuse large B-cell lymphoma, SNHG12, miR-494-3p, CBX3
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