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KMID : 1034820230190010063
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Molecular & Cellular Toxicology
2023 Volume.19 No. 1 p.63 ~ p.70
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Combined treatment of ginsenoside Rg2 and piceatannol mixture reduces the apoptosis and DNA damage induced by UVB in HaCaT cells
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Jeong Seul-A
Chung Yu-Heon
Park So-Jin
Lee Su-Min
Choi Na-Young
Park Jong-Kun
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Abstract
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Background : Ultraviolet B (UVB), a major cause of skin photoaging, leads to DNA damage directly and indirectly in skin cells. DNA damage can contribute to aging by increasing apoptosis, cellular senescence and cell dysfunction. To prevent photoaging and rejuvenate photoaged skin, combinations of active compounds from natural products are used for medicine and cosmetic ingredients.
Objective : In this study, we investigated the synergistic effects of Rg2 and piceatannol (PIC) mixture (RP) on protecting skin against UVB-induced DNA damage in HaCaT cells.
Results : Cells treated with RP for 24 h post UVB exposure increased the cell viability in a concentration-dependent manner compared to non-treated control. In DPPH and DCF-DA assay, RP showed similar free radical scavenging activity to that of single-treatment PIC. RP more decreased the levels of CPD, DDR proteins including p53, p-p53 and p21 and apoptosis-related proteins, such as Bax, Bim, and cleaved caspase-7 than that of single-treatment PIC or Rg2. Also, RP decreased more the mRNA level of MMP-1, -3 and -9 than that of single-treatment PIC or Rg2.
Conclusion : It is inferred that Rg2 promotes DNA repair by regulating genes related with DDR and PIC promotes DNA repair by effectively scavenging UVB-induced ROS through high antioxidant activity. Enhanced DNA repair by RP leads to protecting skin from UVB-induced skin photoaging.
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KEYWORD
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Rg2, Piceatannol, UVB, DNA repair
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