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KMID : 1034820230190010109
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Molecular & Cellular Toxicology
2023 Volume.19 No. 1 p.109 ~ p.117
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LncRNA XIST contributes to epithelial?mesenchymal transformation in posterior opacity via regulating miR-98-5p/COL5A2 axis and PI3K/Akt/FOXO1 pathway
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Junxia Sun
Shasha Han
Ping Chen
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Abstract
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Background : Posterior capsule opacification (PCO), also known as after-cataract, is a special condition in which the cortex of the cataract remains in the pupil area or the formation of a fibrogenic membrane after surgical cataract extraction.
Objective : To explore the effect of lncRNA X inactivation-specific transcript (XIST) on PCO.
Results : First, we proved that XIST was up-regulated in TGF-β2-treated SRA01/04 cells. Inhibition of XIST significantly reduced the proliferation and migration of SRA01/04 cells, and affected the expression of EMT markers. Further research data illustrated that miR-98-5p and COL5A2 were targets of XIST. Besides, pathway enrichment analysis showed that the PI3K/Akt/FOXO1 signaling pathway was associated with XIST.
Conclusion : In the study, we demonstrated that lncRNA XIST contributed to EMT in PCO via regulating miR-98-5p/COL5A2 and PI3K/Akt/FOXO1 pathway, which provided a new treatment strategy for PCO.
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KEYWORD
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Posterior capsule opacification, Epithelial?mesenchymal transformation, Long non-coding RNA, X inactivation-specific transcript, MiR-98-5p, Collagen type V alpha 2 chain, PI3K/Akt/FOXO1
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