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KMID : 1034820230190010135
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Molecular & Cellular Toxicology
2023 Volume.19 No. 1 p.135 ~ p.143
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Angelica dahurica attenuates melanogenesis in B16F0 cells by repressing Wnt/β-catenin signaling
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Chien-Liang Fang
Debakshee Goswami
Chia-Hua Kuo
Cecilia Hsuan Day
Mei-Yi Lin
Tsung-Jung Ho
Liang-Yo Yang
Dennis Jine-Yuan Hsieh
Tzu-Kai Lin
Chih-Yang Huang
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Abstract
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Background : Melanogenesis is a complex process which is tightly regulated by several enzymes. However, abnormal melanogenesis can cause severe dermatological problems. Roots of Angelica dahurica have been used for skin care as a part of traditional Chinese medicine for many generations. However, the role of A. dahurica in melanogenesis remains unclear.
Objective : Previous in vitro and in vivo studies have demonstrated that NK-1R exerts positive effects in melanogenesis via the Wnt/βcatenin signaling pathway. In this study, we investigated the effects of A. dahurica ethanol extract (ADE) on NK-1R and Wnt/β-catenin signaling, and evaluated the effect of NK-1R on melanogenesis in B16F0 cells.
Results : Angelica dahurica ethanol extract efficiently downregulated Neurokinin-1 receptor and Wnt/β-catenin signaling by decreasing the expression of β-catenin, MITF, LEF-1, TYR, TRP1, and TRP2 and increasing the expression of GSK3β, which resulted from the weakened expression of the Neurokinin-1 receptor inhibitor [Sar9,Met(O2)11]-Substance P (SMSP). Furthermore, the intracellular melanin assay and cellular tyrosinase activity confirmed these findings.
Conclusion : This study suggests that ADE has potential to downregulate Neurokinin-1 receptor in SMSP-induced B16F0 cells, thereby repressing the Wnt/β-catenin signaling and reduces melanin production.
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KEYWORD
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Angelica dahurica ethanol extract, [Sar9,Met(O2 )11]-Substance P, Neurokinin-1 receptor, Wnt/β-catenin signaling, Melanogenesis
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