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KMID : 1034820230190010205
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Molecular & Cellular Toxicology
2023 Volume.19 No. 1 p.205 ~ p.217
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Gene expression related to lung cancer altered by PHMG-p treatment in PBTE cells
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Park Yoon-Hee
Jeong Sang-Hoon
Lee Hye-Jin
Kim Cherry
Nam Yoon-Jeong
Kang Ja-Young
Choi Jin-Young
Lee Yu-Seon
Park Su-A
Kim Jae-Young
Park Eun-Kee
Baek Yong-Wook
Lee Hong
Lee Ju-Han
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Abstract
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Background : Humidifier disinfectants (HDs), including polyhexamethylene guanidine phosphate (PHMG-p), are commonly used in Korea to prevent microbial growth. Epidemiological studies show that PHMG-p causes severe lung injury, accompanied by pulmonary inflammation and fibrosis. Several studies have examined humidifier disinfectant-associated lung injuries, but few have examined potential carcinogenic activity of PHMG-p. Our previous studies have shown that PHMG-p can induce lung cancer. However, gene expression changes in squamous cell carcinoma (SCC) caused by PHMG-p have not yet been studied.
Objectives : We aimed to determine the PHMG-p-induced changes in transcription levels of genes involved in SCC development and progression in primary bronchial/tracheal epithelial cells (PBTE cells).
Results : Genetic alterations induced by PHMG-p were designated as fold change (FC) values of greater than 2 or less than 0.5. RNA sequencing analysis revealed that two genes were upregulated and sixteen genes were downregulated in the short-term PHMG-p exposure group. Fourteen genes were upregulated and 3 genes were downregulated in the long-term PHMG-p exposure group.
Conclusion : Our data show that PHMG-p induces genetic changes associated with SCC progression in PBTE cells.
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KEYWORD
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Humidifier disinfectant, Polyhexamethylene guanidine phosphate, Human primary bronchial/tracheal epithelial cells, Total RNA sequencing, Lung squamous cell carcinoma-related genes
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