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KMID : 1034820230190020237
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Molecular & Cellular Toxicology
2023 Volume.19 No. 2 p.237 ~ p.245
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Coal dust exposure induces proliferation and migration of human bronchial epithelial cells
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Amin Li
Yinci Zhang
Ruikai Wang
Ruyue Xu
Yongfang Ma
Li Song
Weiya Cao
Xiaolong Tang
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Abstract
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Background : Coal dust exposure has caused a variety of lung diseases. In addition to genotoxicity and cytotoxicity, other biological changes caused by coal dust (CD) exposure need further study.
Objective : To observe the cellular transformation effects of CD exposure and explore its underlying molecular mechanism, human bronchial epithelial cells (BEAS-2B) were cultured with continuous CD exposure. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay, colony formation assay, wound healing assay, next-generation sequencing (NGS) and western blotting were performed to observe the cell proliferation, migration, genomic transcription and pathological signaling pathways.
Results : We demonstrated that BEAS-2B cells with long-term chronic CD exposure show accelerated proliferation rate and enhanced migration ability, and have altered gene expression profiles and aberrant activation of EGFR/Raf/ERK and PI3K/AKT/mTOR pathways.
Conclusions : The results indicate that chronic CD exposure could induce abnormal proliferation and migration of BEAS-2B cells, lead to the transformation potential of human bronchial epithelial cells.
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KEYWORD
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Coal dust, Human bronchial epithelial cells, Proliferation, Migration, EGFR/Raf/ERK, PI3K/AKT/mTOR
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