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KMID : 1034820230190020293
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Molecular & Cellular Toxicology
2023 Volume.19 No. 2 p.293 ~ p.302
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PCDH8 protects MPP+-induced neuronal injury in SH-SY5Y cells by inhibiting MAPK pathway
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Huaming Wei
Yuping Wu
Huanxia Zhuang
Chung Hwe-Hoon
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Abstract
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Background : Parkinson's disease (PD) is a common degenerative disease of the nervous system in the elderly.
Objectives : To investigate the effect of protocadherin 8 (PCDH8) on PD and explore the underlying mechanism.
Results : PCDH8 was down-regulated in PD. Silencing PCDH8 inhibited cell viability, increased cell cytotoxicity and apoptosis, but overexpression of PCDH8 acted the opposed effects. Then, silencing PCDH8 increased the levels of IL-1β, IL-8, TNF-α and ROS production, but decreased SOD and CAT activity, while up-regulation of PCDH8 acted the opposed effects. In addition, down-regulation of PCDH8 activated MAPK pathway, but up-regulation of PCDH8 inhibited MAPK pathway. Furthermore, in SH-SY5Y cells treated by MPP+, SB203580 reversed cell injuries induced by silencing PCDH8.
Conclusion : PCDH8 was down-regulated in PD, and PCDH8 protected MPP+-induced neuronal injury in SH-SY5Y cells via inhibiting MAPK pathway.
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KEYWORD
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PCDH8, Parkinson′s disease, MAPK pathway, Inflammatory factors
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