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KMID : 1034820230190020343
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Molecular & Cellular Toxicology
2023 Volume.19 No. 2 p.343 ~ p.352
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LncRNA PVT1 upregulates FBN1 by sponging miR-30b-5p to aggravate pulpitis
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Yuanyuan Li
Shuangshuang Li
Ruijing Li
Huilin Xu
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Abstract
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Background : Pulpitis is a disease mainly caused by bacterial infection. Long noncoding RNAs (lncRNAs) influence the advancement of pulpitis. In this paper, we reconnoitered the characters of lncRNA plasmacytoma variant translocation 1 (PVT1) in pulpitis.
Objective : PVT1, microRNA-30b-5p (miR-30b-5p) and fibrillin 1 (FBN1) levels were identified by qRT-PCR and western blot. In addition, the cell functions were scrutinized. Additionally, the link between miR-30b-5p and PVT1 or FBN1 was identified by dual-luciferase reporter assay.
Result : The contents of PVT1 and FBN1 were augmented, but the miR-30b-5p level was declined in pulpitis. Knockdown of PVT1 enhanced cell proliferation and cell vitality, whereas inhibited cell inflammatory reaction in HDPFs under LPS stimulation. In mechanism, PVT1 acted as a miR-30b-5p sponge to adjust the content of FBN1. Moreover, miR-30b-5p mediated LPS-mediated repression of proliferation and promotion of inflammatory reaction HDPFs by FBN1.
Conclusion : PVT1 expedited the improvement of pulpitis via miR-30b-5p/FBN1.
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KEYWORD
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Pulpitis, PVT1, miR-30b-5p, FBN1
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