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KMID : 1034820230190020363
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Molecular & Cellular Toxicology
2023 Volume.19 No. 2 p.363 ~ p.371
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MicroRNA-5582-3p alleviates the progression of osteosarcoma via targeting FZD4
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Wanming Qu
Hongbin Zhou
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Abstract
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Background : Dysfunction of microRNAs (miRNAs) is a common characteristic during cancer progression. The suppressive function of miR-5582-3p in cancers has been emerged in recent studies, however, the clinical value and novel targets of miR-5582-3p in osteosarcoma (OS) have not been identified.
Objective : Investigate the function and underlying mechanism of miR-5582-3p in OS.
Results : miR-5582-3p expression was significantly reduced in OS tissues and cells. Lower level of miR-5582-3p had closed relationship with the advanced development and poor 5-year overall survival of OS patients. Transfection of miR-5582-3p in OS cells obviously inhibited the cell proliferation, invasion and colony formation. Highly expressed miR-5582-3p also delayed the cell cycle progression and promoted cell apoptosis. Furthermore, FZD4 was validated as a target of miR-5582-3p. miR-5582-3p interacted with the 3′-untranslated regions (3′-UTR) of FZD4 and reduced FZD4 expression in OS cells. Expression of miR-5582-3p was inversely correlated with that of FZD4 in OS tissues. Transfection of FZD4 rescued the suppressed OS cell proliferation, attenuated miR-5582-3p induced cell apoptosis and invasion.
Conclusions : Our findings uncovered the novel anti-cancer potency of miR-5582-3p in OS via repressing FZD4, suggesting the possibility of miR-5582-3p as a therapeutic target of OS.
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KEYWORD
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miR-5582-3p, FZD4, Osteosarcoma, Cell proliferation
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