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KMID : 1034820230190030473
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Molecular & Cellular Toxicology
2023 Volume.19 No. 3 p.473 ~ p.481
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NEAT1 promotes keratinocyte migration and proliferation during wound healing by regulating miR-26a-5p/LGR4 axis
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Lan Zhang
Rong Tian
Kui Wang
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Abstract
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Background : Wound re-epithelialization is considered as an extremely important link in the complete reconstruction of the epidermal barrier. The present study is aimed to determine the association between transforming growth factor β (TGF-β) and re-epithelialization.
Objective : It was aimed to explore the possible molecular mechanism of TGF-β mediated re-epithelialization.
Results : NEAT1 was upregulated in TGF-β1-treated HaCaT cells and promoted cell proliferation. NEAT1 overexpression enhanced the wound healing and upregulated MMP-2 and MMP-9 in TGF-β1-treated HaCaT cells. Mechanically, TGF-β1 down-regulated miR-26a-5p through targeting NEAT1 in HaCaT cells. Furthermore, NEAT1/miR-26a-5p axis regulated the expression of LGR4 in HaCaT cells. Finally, the results showed that NEAT1/miR-26a-5p/LGR4 axis was involved in TGF-β1-mediated re-epithelialization.
Conclusion : NEAT1/miR-26a-5p/LGR4 network is an important participant in TGF-β1-mediated keratinocyte proliferation and migration, which provides a novel perspective for understanding the cellular behavior and related molecular events in re-epithelialization.
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KEYWORD
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Transforming growth factor β, Nuclear paraspeckle assembly transcript 1, miR-26a-5p, Leucine-rich repeat-containing G-protein-coupled receptor 4, Re-epithelialization
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