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KMID : 1034820230190030483
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Molecular & Cellular Toxicology
2023 Volume.19 No. 3 p.483 ~ p.490
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Iridin abrogates LPS-induced inflammation in L6 skeletal muscle cells by inhibiting NF-κB and MAPK signaling pathway
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Pritam Bhagwan Bhosale
Ha Sang-Eun
Kim Hun-Hwan
Abuyaseer Abusaliya
Park Min-Yeong
Kim Gon-Sup
Kim Jin-A
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Abstract
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Background : Iridin is a glycoside form of flavonoids isolated from Belamcanda chinensis, and Iris florentina has numerous biological properties, including antitumor, antiproliferative, anti-inflammatory and antioxidant properties. Inflammatory diseases are an essential health concern and have a growing incidence worldwide, so developing safe drugs remains an important objective.
Objective : In the present study, we evaluated iridin's anti-inflammatory effect on LPS-stimulated skeletal muscle cells (L6). The anti-inflammatory action of iridin elucidated through NF-κB (nuclear factor-kappa B) and MAPK (mitogen-activated protein kinases) pathway in LPS-stimulated L6 cells.
Results : Determination of cytotoxicity was examined by MTT assay. The expression level of cyclooxygenase?2 (COX-2) and nitric oxide synthase (iNOS) was evaluated by western blot analysis. To elucidate the underlying mechanism, NF-κB and MAPKs proteins were investigated by immunoblot. Iridin could decrease the phosphorylation of NF-κB and MAPK proteins in LPS-induced L6 cells.
Conclusion : The results showed that iridin inhibited the phosphorylation of NF-κB and MAPK proteins and inflammatory cytokines COX-2 and iNOS in LPS-induced L6 cells. Based on findings in this work, iridin possesses anti-inflammatory action on L6 cells and could be considered for an attractive candidate for anti-inflammation.
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KEYWORD
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iNOS, COX-2, NF-κB, MAPK, And Iridin
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