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KMID : 1034820230190030521
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Molecular & Cellular Toxicology
2023 Volume.19 No. 3 p.521 ~ p.529
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Concurrent upregulation of immune checkpoint molecule genes in colorectal cancer
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Bang Hui-Jae
Sohn Joon-Hyung
Kim Soo-Ki
Kim Cheol-Su
Cho Mee-Yon
Lee Shin-Hee
An Sang-Hyun
Kim Kwang-Min
Kim Youngwan
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Abstract
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Background : A simultaneous gene expression pattern of immune checkpoint molecules might exist in colorectal cancer. This hypothesis has rarely been tested in human in vivo samples.
Objective : We investigated the gene expression patterns of immune checkpoint molecules in human colorectal cancer tissues using quantitative polymerase chain reaction (qPCR) with a focus on concurrent gene expression.
Results : We included 14 females and 16 males with a mean age was 68.5 years. The mean number of all immune checkpoint molecules did not differ significantly between normal and tumor tissues. Histological grade 3 tumors were more common in the PDCD1-expressing group [3 (25.0%) vs. 0 (0%) (p?=?0.042)]. All six and four immune checkpoint molecules were expressed in eight and three PDCD1-positive patients, respectively. Specifically, CD274 was expressed in 11 of 12 PDCD1-positive patients, while LAG3 and IDO1 were expressed simultaneously in all patients expressing CD274.
Conclusion : Colorectal cancers more commonly express multiple immune checkpoint molecules simultaneously than single molecules. This suggests that blocking multiple immune checkpoint pathways may serve as a potential strategy for immunotherapy.
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KEYWORD
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Colorectal cancer, Immune checkpoint inhibitors, Polymerase chain reaction
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