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KMID : 1034820230190030539
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Molecular & Cellular Toxicology
2023 Volume.19 No. 3 p.539 ~ p.549
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Beta-naphthoflavone increases the differentiation of osteoblasts and suppresses adipogenesis in human adipose derived stem cells involving STAT3 pathway
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Ming Lu
Min Li
Tao Luo
Kang Woong-Chol
Mingxin Wang
Huashi Xue
Kwon Ki-Hwan
Qiu Chen
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Abstract
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Background : Treating large-volume bone defects (LVBD) remains a challenge for orthopedics and maxillofacial surgeons globally.
Objective : The present study was to aimed investigate the role of Beta-naphthoflavone (BNF) a synthetic flavonoid on differentiation of osteoblast of human adipose derived stem cells.
Results : BNF at 1 μM showed a significantly increased ALP activity and rate of cell proliferation compared to control on the 3rd, 7th and 14th day. Also, it was observed that, BNF at 1 μM resulted in significantly increased expression of BSP. On the 14th day of treatment, BNF at all the three treatments resulted in increased levels of OCN. Also, extracellular matrix mineralization levels were recorded to be highest for BNF 1 μM. The levels of Runx2 were higher in 1 μM treated group on the 3rd day compared to other, whereas in contrast the levels were lower on the 7th and 14th day compared to control. BNF at all the three treatments caused a significant decrease in levels of pSTAT3, C/EBP-α and PPAR-γ levels.
Conclusion : BNF treatment resulted in increased differentiation of osteoblast of hASCs by targeting STAT3 pathway.
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KEYWORD
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Beta-Napthoflavone, hASCs, Osteoblast, Bone regeneration, STAT3
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