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KMID : 1034820230190030591
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Molecular & Cellular Toxicology
2023 Volume.19 No. 3 p.591 ~ p.599
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Matrine promotes trophoblast invasion and reduces inflammation via miR-19a-3p to prevent preeclampsia
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Weina Yang
Qian Kang
Chunlei Li
Shuchun Bo
Oh Yeon-Mok
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Abstract
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Background : During preeclampsia (PE), reactive oxygen species (ROS) production and inflammation are increased. Matrine exerts diverse pharmacological effects, including anti-inflammatory and anti-apoptotic effects.
Objective : The present study aimed to study the therapeutic effects of matrine on PE. Human trophoblasts (HTR-8/SVneo cells) were treated with H2O2 to simulate oxidative stress, followed by treatment with various concentrations of matrine. Next, flow cytometry was used to examine apoptosis. Cell invasion and migration were examined by Transwell and scratch wound assays, respectively. The concentrations of IL-6, IL-8, and TNF-α were quantified by enzyme-linked immunosorbent assays. The expression levels of miR-19a-3p and ZEB2 were quantified by qRT-PCR. The interaction between miR-19a-3p and ZEB2 was examined by a dual-luciferase reporter assay.
Result : Matrine attenuated H2O2-induced increases in ROS levels, apoptosis, and cytokine release in HTR/SVneo cells. Matrine also increased cell migration and invasion during oxidative stress by reducing miR-19a-3p expression. Moreover, miR-19a-3p can target ZEB2 directly.
Conclusion : During oxidative stress, matrine can reduce ROS levels, apoptosis, and inflammation in human trophoblasts and promote migration and invasion via miR-19a-3p, offering a potential new approach for the treatment of PE.
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KEYWORD
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Apoptosis, Inflammation, Invasion, Matrine, miR-19a-3p, Migration, Oxidative stress, Preeclampsia, ZEB2
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