|
KMID : 1094719970020020141
|
|
Biotechnology and Bioprocess Engineering
1997 Volume.2 No. 2 p.141 ~ p.146
|
|
|
Immobilization ofPhotobacterium phosphoreum for monitoring of toxic substances
|
|
Chun Uck-Han
Kim Jung-In
Yoo Seung-O
Lee Hong-Joo
|
|
|
Abstract
|
|
|
|
A new sensing system based on the immobilization of luminescent bacteria,Photobacterium phosphoreum, was proposed for continuous real-time monitoring of pollutants. The response curves demonstrate thatPhotobacterium phosphoreum immobilized on the strontium alginate was very sensitive to seven reference chemicals used. The significant inhibitory concentrations for bioluminescence emission were 5 ppm for Pb(NO3)2, NiCl2, CdCl2, 50 ppm for NaAsO2, 0.1 ppm for HgCl2, 0.5 ppm for pentachlorophenol and less than 5 ppm for SDS, respectively. The alginate mixed-cells (AMC) retained their luminescence during experimental period (29 days) under storage condition of ? 80°C. The variables affecting performance of continuous flow through monitoring (CFTM) were optimized in order to ensure stability and efficiency. The flow through cell with strontium-alginate immobilized luminescent bacteria was tested with salicylate and 4-nitrophenol and a rapid response of luminescence was recorded by time drivemode in bioluminescence spectrometer after exposure to both toxicants.
|
|
|
KEYWORD
|
|
|
bioluminescence, photobacterium, immobilization
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|