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KMID : 1094719980030010048
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Biotechnology and Bioprocess Engineering
1998 Volume.3 No. 1 p.48 ~ p.49
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Efficient transformation ofKlebsiella oxytoca by electroporation
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Jeong Ki-Jun
Lee Hyun-Sook
Lee Sang-Yup
Chang Yong-Keun
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Abstract
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A protocol for the transformation ofKlebsiella oxytoca by electroporation was developed. Preparation of competent cells at early exponential phase was most critical to obtain a high transformation efficiency. The highest efficiency of 1.6 × 106 transformants per μg DNA (pBR 322) could be obtained by electroporation ofK. oxytoca cells prepared at the OD600 of 0.2 with 1.25 μg DNA at the filed strength of 2.5 kV, the parallel resistance of 200 Ω and capacitance of 25 μF.
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KEYWORD
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Klebsiella oxytoca, electroporation, efficiency
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