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KMID : 1094719990040020147
Biotechnology and Bioprocess Engineering
1999 Volume.4 No. 2 p.147 ~ p.147
Extracellular protease production fromXenorhabdus nematophilus, a symbiotic bacterium of entomopathogenic nematodes
Ryu Keun-Garp

Bae Jun-Sang
Park Sun-Ho
Abstract
Effects of nutrients and growth temperature on the production of an extracellular protease fromXenorhabdus nematophilus, a symbiotic bacterium of entomopathogenic nematodes were studied for batch culture. Tryptone and fructose were most effective nitrogen and carbon sources to produce high level of the protease within 24?28 hr of incubation. The stability of protease was poor during the incubation of the bacteria. The protease activity increased in parallel with cell growth then decreased significantly for extended culture periods of the bacteria over 48 hr at 22?30¡ÆC. Autolysis of the protease was not a major cause for the decreased protease activity since no decrease in the protease activity was observed for the whole culture broth of the bacteria which was stored up to 80 hr at 4¡ÆC.
KEYWORD
entomopathogenic nematodes, symbiotic bacteria, protease, carbon source, nitrogen source
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