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KMID : 1094719990040030224
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Biotechnology and Bioprocess Engineering
1999 Volume.4 No. 3 p.224 ~ p.225
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Concentrating rotavirus and recombinant enhanced green fluorescent protein fromE. coli using a temperature-sensitive hydrogel
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Chang Kyung-Hwa
Park Jong-Hwa
Park Chang-Ho
Chung In-Sik
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Abstract
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Recombinant enhanced green fluorescent protein (EGFP) fromE. coli was concentrated 1.9 times by ultrafiltration using a temperature-sensitive hydrogel. Protein recovery and separation efficiency were 64% and 45%, respectively. Increased concentration of recombinant EGFP was confirmed by SDS-PAGE. Rotavirus was concentrated 3.2 times by ultrafiltration using a temperature-sensitive hydrogel, at 95% of virus recovery and 93% of separation efficiency. Hydrogel ultrafiltration appears to be an attractive alternative for the concentration of rotavirus and recombinant proteins fromE. coli.
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KEYWORD
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temperature sensitive hydrogel, recombinant enhanced green fluorescent protein, rotavirus, ultrafiltration
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