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KMID : 1094720180230020183
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Biotechnology and Bioprocess Engineering
2018 Volume.23 No. 2 p.183 ~ p.193
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Enhanced Production of β-D-glycosidase and α-L-arabinofuranosidase in Recombinant Escherichia coli in Fed-batch Culture for the Biotransformation of Ginseng Leaf Extract to Ginsenoside Compound K
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Kim Tae-Hun
Yang Eun-Joo
Shin Kyung-Chul
Hwang Kyeong-Hwan
Park Jun-Seong
Oh Deok-Kun
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Abstract
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Ginsenoside compound K is an essential ingredient in nutritional supplements, cosmetics, and traditional medicines. However, cultivation for the production of enzymes involved in ginsenoside biotransformation has not been attempted in a fermenter. The host strain Escherichia coli ER2566 and the constitutive pHCE vector were selected for the efficient production of β-D-glycosidase, and expression medium composition to produce Sulfolobus solfataricus β-glycosidase expressed in E. coli was optimized in flask and batch cultures. The total activity of β-Dglycosidase in fed-batch culture using a fermenter increased 14-fold before optimization. S. solfataricus β-D-glycosidase and Thermotoga petrophila α-L-arabinofuranosidase were produced in a fed-batch culture. These two enzymes completely converted protopanaxadiol-type ginsenosides in ginseng leaf extract obtained from discarded ginseng leaves as a renewable substrate to compound K. The effective bioprocess for compound K production developed here will contribute to the industrial biological production of compound K.
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KEYWORD
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β-D-glycosidase, α-L-arabinofuranosidase, fedbatch culture, ginseng leaf, ginsenoside compound K
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