KMID : 1098420150230050357
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Korean Journal of Medicinal Crop Science 2015 Volume.23 No. 5 p.357 ~ p.362
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Production of Adventitious Root and Analysis of Effective Components from in vitro Culture of Astragalus membranaceus
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Hur Mok
Lee Dae-Young Lee Jeong-Hoon Ahn Tae-Jin Lee Jeong-Hoon Kim Young-Guk Cha Sun-Woo Um Yu-Rry
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Abstract
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Background : A series of studies were conducted to optimize adventitious root induction in vitro from explants of Astragalus membranaceus using various nutrient media supplemented with plant hormones.
Methods and Results : Levels of active components were analyzed from adventitious roots induced under different media conditions. Among the different media conditions, Murashige and Skoog medium supplemented with 1.0 §· ? §¤ ?1 indole-3-butyric acid resulted in the greatest adventitious root induction rate. The amount of the major active component of the adventitious roots of Ama1, calycosin-7-O-¥â-D-glucopyranoside was higher than that of other adventitious root samples.
Conclusions : These results suggest that the adventitious roots of A. membranaceus could be used for the commercial production of medicines.
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KEYWORD
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Astragalus membranaceus, Adventitious Roots, Calycosin-7-O-${\beta}$ ¼ö½Ä À̹ÌÁö-D-Glucopyranoside
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