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KMID : 1098420170250020108
Korean Journal of Medicinal Crop Science
2017 Volume.25 No. 2 p.108 ~ p.114
Investigation of Pathogenic Microbial Contamination in Medicinal Herb Products on the Market
Ham Hee-Jin

Yoo In-Sil
Lee Jib-Ho
Kim Soo-Jin
Yoo Young-Ah
Lee Eun-Soon
Kim Hee-Sun
Abstract
Background: The study was conducted to investigate the distributions of faecal bacteria in commercial oriental medicine herb products.

Methods and Results: A survey was conducted on the microbial contamination levels and antimicrobial specificity of Bacillus cereus and other microbes using 106 oriental medicine herb products on sale in Seoul. Pouring and isolation methods such as standard plate counts were used to identify the bacteria. The isolated bacterias included coliforms, Bacillus spp., Enterococcus spp., Staphylococcus spp., Listeria spp.were identified by using gram staining and an API (analytical profile index) kit. Antimicrobial drugs discs were determined by CLSI (clinical and laboratory standards institute).

Conclusions: The bacterial isolates present in the herbal medicines included 98 coliforms, 45 Bacillus spp., 29 Enterococcus spp., and 2 Listeria spp. Among these, there were nine Bacillus cereus strains, one Enterococcus faecium strain, and one Enterococcus faecalis strain present. The 9 Bacillus cereus strains were tested for susceptibility to 36 types of antibiotics products by the disc diffusion method. The strains showed resistance to 13 of these antibiotic products and semi-resistance to 5 antibiotic products. On the basis of these results, any oriental medicine herb product can be assumed to be contain resistant or semi-resistant bacterial strains. Therefore, we suggest prescribing guidelines and special management for the use of antibiotics in farms producing oriental medicine herb products.
KEYWORD
Bacillus cereus, Antimicrobial Specificity, Oriental Medicines
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