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KMID : 1100720120320050359
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Annals of Laboratory Medicine
2012 Volume.32 No. 5 p.359 ~ p.361
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Multiplex PCR for Rapid Detection of Genes Encoding Class A Carbapenemases
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Hong Sang-Sook
Kim Kyeong-Mi
Huh Ji-Young
Jung Bo-Chan
Kang Myung-Seo
Hong Seong-Geun
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Abstract
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In recent years, there have been increasing reports of KPC-producing Klebsiella pneumoniae in Korea. The modified Hodge test can be used as a phenotypic screening test for class A carbapenamase (CAC)-producing clinical isolates; however, it does not distinguish between carbapenemase types. The confirmation of type of CAC is important to ensure optimal therapy and to prevent transmission. This study applied a novel multiplex PCR assay to detect and differentiate CAC genes in a single reaction. Four primer pairs were designed to amplify fragments encoding 4 CAC families (SME, IMI/NMC-A, KPC, and GES). The multiplex PCR detected all genes tested for 4 CAC families that could be differentiated by fragment size according to gene type. This multiplex PCR offers a simple and useful approach for detecting and distinguishing CAC genes in carbapenem-resistant strains that are metallo-β-lactamase nonproducers.
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KEYWORD
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Carbapenemase, Multiplex PCR, KPC, GES
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