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KMID : 1100820160060020111
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Laboratory Medicine Online
2016 Volume.6 No. 2 p.111 ~ p.115
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Bacteroides nordii and Bacteroides salyersiae Isolated from Post-operative Peritonitis Patients
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Yoon Ji-Hoon
Kim Hyun-Soo
Lee Yang-Soon
Kim Myung-Sook
Yong Dong-Eun
Jeong Seok-Hoon
Lee Kyung-Won
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Abstract
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In this study, we report three cases in which two species of the Bacteroides fragilis group, 'Bacteroides nordii' and 'Bacteroides salyersiae', were isolated from peritoneal fluid cultures from post-operative peritonitis patients. The two species of the B. fragilis group were initially misidentified as B. fragilis/Bacteroides stercoris and Bacteroides ovatus by Rapid ID 32A (bioMe rieux, France), and finally confirmed as B. nordii and B. salyersiae using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16s rRNA sequencing. For the identification of anaerobes, particularly B. fragilis group organisms, MALDI-TOF MS is a useful method not only because of its concordance with 16S rRNA sequencing results, but also because of its rapidity and simple procedure.
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KEYWORD
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Bacteroides fragilis group, Bacteroides nordii, Bacteroides salyersiae, Spectrometry, Mass, Matrix-assisted laser desorption-ionization
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