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KMID : 1100820180080010015
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Laboratory Medicine Online
2018 Volume.8 No. 1 p.15 ~ p.18
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Comparison of Interferon-gamma Secretion by Stimulated NK Cells and T cells from Healthy Subjects
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An Gyu-Dae
Kim Kyeong-Hee
Lim Hyeon-Ho
Kim Min-Chan
Lee Sang-Yeob
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Abstract
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Interferon-γ (IFN-γ) is an important cytokine produced by natural killer (NK) cells and T cells in response to various stimuli. The levels of IFN-γ secreted after stimulation of NK cells using a recombinant cytokine is represented as one of functions of NK cells. Recently, a method for evaluating NK cell activity in whole blood samples was developed. The levels of IFN-γ secreted after NK cell stimulation with PROMOCA™ (ATGen, Korea) and T cell stimulation with phytohemagglutinin (PHA) were compared using two different commercial kits: NK Vue Gold (ATGen, Korea) and QuantiFERON-TB Gold In-Tube (Cellestis, Australia). Participants included 43 healthy individuals. Whole blood samples were incubated with either PROMOCA, a recombinant cytokine that specifically activates NK cells, or with PHA. IFN-γ levels in the supernatants were measured by ELISA. The level of IFN-γ by PROMOCA stimulation (PROMOCA IFN-γ) was more varied than that by stimulation with PHA (PHA IFN-γ) (median 1,544.4 pg/mL [ range 193.7?2,530.9] vs. median 2,470.1 pg/mL [ 2,250.1?2,874.4] P=0.0001). The median of PHA IFN-γ/PROMOCA IFN-γ ratio was 1.9 (1.1?12.4). There was a significant difference in levels of IFN-γ secreted after stimulation with PROMOCA or PHA in the healthy population.
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KEYWORD
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Natural killer cells, Interferon-gamma, Phytohemagglutinin, ELISA, PROMOCA
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