KMID : 1100820180080040140
|
|
Laboratory Medicine Online 2018 Volume.8 No. 4 p.140 ~ p.147
|
|
Clinical Usefulness of ImmuneCheck¢â IgG for Rapid Semiquantitation of Total IgG
|
|
Choi Jin-Young
Lee Sang-Chul Park Kyung-Hee Yoon Hey-Lyeong Kim Hyon-Suk Park Jung-Won
|
|
Abstract
|
|
|
Background: Conventional IgG assays require costly equipment and skilled experts. Semiquantitative measurement of total IgG using point-of-care testing devices may be the solution for these limitations. This study evaluated the reproducibility of the ImmuneCheck¢â IgG assay (ProteomeTech Inc., Korea) and the correlation of its results with conventional laboratory IgG results in the serum and whole blood.
Methods: Both the serum and whole blood samples from 120 patients were used. To evaluate the intra-test reproducibility and inter-test correlation, intraclass correlation coefficient (ICC) analysis was used.
Results: The concentration of serum total IgG measured by cobas¢ç 6000 (Roche Diagnostics, Switzland) ranged from 690.4 to 2,756.4 mg/dL. The intra-test reproducibility of ImmuneCheck¢â IgG was high (Serum ICC=0.724, P<0.001; Whole blood ICC=0.843, P<0.001). The inter-test correlation between the ImmuneCheck¢â IgG and cobas¢ç 6000 results was very good (Serum ICC=0.805, P<0.001; Whole blood ICC=0.842, P<0.001). Because there were no samples with a total IgG level lower than 600 mg/dL, the pre-existing serum samples were diluted and then the linearity tests were conducted. The intra-test reproducibility for the diluted serum samples was almost perfect (ICC=0.995, P<0.001), and the inter-test correlation between the ImmuneCheck¢â IgG and cobas¢ç 6000 results was also strong (ICC=0.992, P<0.001).
Conclusions: The ImmuneCheck¢â IgG assay is reproducible and highly correlated with the conventional IgG assay for the serum and whole blood. It could be applied for the rapid detection of total IgG.
|
|
KEYWORD
|
|
Total IgG POCT, Rapid semiquantitation, Total IgG assay
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|