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KMID : 1100820200100040326
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Laboratory Medicine Online
2020 Volume.10 No. 4 p.326 ~ p.329
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A Case of Partial D Type VI-3 Confirmed by RHD Genotyping
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Lee Jong-Kwon
Yu Hong-Bi
Chung Yoo-Na
Bae Jae-Chun
Cho Duck
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Abstract
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Weak D and partial D result in quantitative and qualitative changes in RhD protein expression respectively. It is difficult to discriminate weak D from partial D by serological tests alone. RHD genotyping is a useful method that complements serological results. A 64-year-old woman visited our hospital for microvascular decompression surgery. Her blood type was O, D negative by manual tube test and as per auto analyzer results (QWALYS-3 system; DIAGAST, France). Weak D and partial D tests were performed by using two different monoclonal anti-D reagents (Bioscot; Merck Millipore, UK; Bioclone; Ortho Clinical Diagnostics, USA) and a panel of nine monoclonal antibodies, including anti-D IgM and IgG (D-Screen; DIAGAST, France). However, these serological tests could not confirm the subtype of partial D. Therefore, sequencing of RHD exon 1 to 10 was additionally performed for the patient and the case was revealed to be partial DVI type 3.
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KEYWORD
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RhD, Weak D, Partial D, Genotyping, Sequencing
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