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KMID : 1100820210110040290
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Laboratory Medicine Online
2021 Volume.11 No. 4 p.290 ~ p.296
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Evaluation of the AccuPower® RV1 Real-Time RT-PCR Kit and the AccuPower® RV1 Multiplex Kit for SARS-CoV-2 and Influenza Virus Detection
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Kim Man-Jin
Park Hyun-Woong
Jeon You-La
Shin Ho-Seob
Cho Sung-Im
Kim Bo-Ram
Lee Jee-Soo
Park Sung-Sup
Seong Moon-Woo
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Abstract
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Background: The AccuPower® RV1 Real-Time RT-PCR Kit (Bioneer, Korea) and AccuPower® RV1 Multiplex Kit (Bioneer) are one-step real-time reverse transcription PCR assays for detecting severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and influenza A and B.
Methods: We clinically evaluated the AccuPower® RV1 Real-Time RT-PCR Kit and AccuPower® RV1 Multiplex Kit by comparing their results for 1,098 clinical samples. The presence of SARS-CoV-2 was confirmed using the Allplex™ 2019-nCoV Assay (Seegene, Korea) and Standard M nCoV Real-Time Detection Kit (SD Biosensor, Korea). Influenza viruses were detected using the Allplex™ Respiratory Panel 1 (Seegene).
Results: The comparative positive and negative agreement values of the AccuPower® RV1 Real-Time RT-PCR Kit for SARS-CoV-2 and influenza A and B were 100%. The positive agreement of the AccuPower® RV1 Multiplex Kit was 100% for SARS-CoV-2 and 98.77% for influenza A and B. The kappa values for SARS-CoV-2 and influenza A and B were >0.99. SARS-CoV-2 was evaluated using both sputum and nasopharyngeal or oropharyngeal swabs. There was no difference in the detection rates for each type.
Conclusions: The findings confirm the clinically comparable performances of the AccuPower® RV1 Real-Time RT-PCR Kit and the AccuPower® RV1 Multiplex Kit.
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KEYWORD
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SARS-CoV-2, Influenza virus, Multiplex real-time PCR, Clinical evaluation
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