|
KMID : 1120220110020030216
|
|
Osong Public Health and Research Perspectives
2011 Volume.2 No. 3 p.216 ~ p.217
|
|
|
A Contribution of MdfA to Resistance to Fluoroquinolones in Shigella flexneri
|
|
Kim Jun-Young
Jeon Se-Mi
Kim Hyung-Jun
Park Mi-Sun
Kim Seong-Han
|
|
|
Abstract
|
|
|
|
In this study, we measured the drug resistance conferred by mdfA mutations in two Shigella flexneri strains. A mutant in mdfA genes was constructed by polymerase chain reaction?based, one-step inactivation of chromosomal genes. The antimicrobial susceptibility of parent and mutant strains to fluoroquinolones was determined by minimal inhibitory concentration (MICs). The △mdfA mutants were somewhat more susceptible to fluoroquinolones than the parent strains. The low level changes in MICs of the △mdfA mutants suggest that mdfA contributed the fluoroquinolone resistance in S flexneri. This finding found that the increased expression level of an MdfA efflux pump mediated fluoroquinolone resistance, but it is not likely a major effecter of higher resistance levels.
|
|
|
KEYWORD
|
|
|
efflux pump, fluoroquinolones resistance, MdfA, Shigella flexneri
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|