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KMID : 1120220120030020068
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Osong Public Health and Research Perspectives
2012 Volume.3 No. 2 p.68 ~ p.73
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Effect of Maternal Immune Status on Responsiveness of Bacillus Calmette-Gurin Vaccination in Mouse Neonates
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Choi Jong-Su
Kim Ryang-Yeo
Rho Se-Mi
Ewann Fanny
Mielcarek Nathalie
Song Man-Ki
Czerkinsky Cecil
Kim Jae-Ouk
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Abstract
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Objectives: Bacillus Calmette-Guerin (BCG) vaccination has proven to be efficient in immunologically naive infants; however, it has not been investigated that maternal natural exposure to Mycobacterium and/or BCG vaccine could influence the characteristics of immune responses to BCG in newborns. In this study, we analyzed whether the maternal immune status to M tuberculosis (M tb) can affect neonatal immunity to BCG using a mouse model.
Methods: Neonates were obtained from mice that were previously exposed to live BCG, to live M avium, or to heat-killed M tb H37Rv, and from naive control mothers. One week after birth, the neonates were divided into two subgroups: one group immunized with live BCG via the subcutaneous route and the other group of neonates sham-treated. Interferon-gamma (IFNγ) secretion in response to in vitro stimulation with heat-killed BCG or purified protein derivative (PPD) was examined. Protection against M tb infection was evaluated by challenging mice nasally with live M tb H37Rv followed by counting colonies from spleen and lung homogenates.
Results: BCG-immunized neonates showed increased IFNγ secretion in response to heat-killed BCG or PPD. All mice in BCG-immunized neonates subgroups showed reduced bacterial burden (colony forming unit) in the lungs when compared with control naive neonate mice. However, no statistically significant difference was observed when comparing BCG-immunized mice born from mothers previously exposed to M avium or immunized with either heat-killed H37Rv or live BCG and mice born from naive mothers.
Conclusion: The maternal immune status to M tb does not appear to impact on the immunogenicity of BCG vaccine in their progeny in our experimental conditions.
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KEYWORD
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BCG, maternal effect, Mycobacterium tuberculosis, neonates, tuberculosis
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