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KMID : 1120220120030020113
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Osong Public Health and Research Perspectives
2012 Volume.3 No. 2 p.113 ~ p.117
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Multiplex Real-Time Polymerase Chain Reaction-Based Method for the Rapid Detection of gyrA and parC Mutations in Quinolone-Resistant Escherichia coli and Shigella spp.
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Kim Jun-Young
Jeon Se-Mi
Kim Hyung-Jun
Park Mi-Sun
Kim Soo-Bok
Kim Seong-Han
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Abstract
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Two real-time polymerase chain reaction assays were developed to detect mutations in codons 83 and 87 in gyrA and in codons 80 and 91 in parC, the main sites that causes quinolone resistance in pathogenic Escherichia coli and Shigella spp. isolates. These assays can be employed as a useful method for controlling infections caused by quinolone-resistant E coli and Shigella isolates.
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KEYWORD
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fluoroquinolone, gyrA, multiplex real-time PCR, parC
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