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KMID : 1120220130040060342
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Osong Public Health and Research Perspectives
2013 Volume.4 No. 6 p.342 ~ p.346
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Development of a Specific and Rapid Diagnostic Method for Detecting Influenza A (H1N1) pdm09 Virus Infection Using Immunochromatographic Assay
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Ji Mi-Jung
Cho Byung-Ki
Cho Young-Shik
Choi Young-Jin
Kwon Dong-Hyok
Shin Kyeong-Cheol
Lee Joo-Yeon
Kang Chun
Yoon Byoung-Su
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Abstract
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Objectives: The aim of this study was to develop an immunochromatographic assay (ICA) for the detection of influenza A (H1N1) pdm09 virus infection.
Materials and methods: Several monoclonal antibodies against influenza A (H1N1) pdm09 virus were generated and an ICA (pdm09-ICA) was developed for the rapid and specific detection of influenza A (H1N1) pdm09 virus infection. The specificity and sensitivity of the developed assay were compared with that of hemagglutination assay and real-time reverse-transcription polymerase chain reaction (rRT-PCR).
Results: The detection limit was estimated to be 1/2 (8) hemagglutinating unit; the sensitivity and specificity rates of pdm09-ICA were 75.86% (110/145) and 100% (43/43), respectively, compared with rRT-PCR. The cross-reactivity for 20 influenza viruses, including seasonal H1N1 viruses, was found to be negative except for the H1N1 virus (A/Swine/Korea/GC0503/2005).
Conclusion: These results indicate that the proposed method can be easily used for rapid and specific detection of the pdm09 infection. The assay developed in this study would be a useful tool for distinguishing the pdm09 infection from seasonal influenza A and B infections.
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KEYWORD
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immunochromatographic assay, immunochromatography test, influenza, pdm09
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