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KMID : 1120220140050050245
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Osong Public Health and Research Perspectives
2014 Volume.5 No. 5 p.245 ~ p.250
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Molecular Investigation of Quinolone Resistance of Quinolone Resistance-Determining Region in Streptococcus pneumoniae Strains Isolated from Iran Using Polymerase Chain Reaction?Restriction Fragment Length Polymorphism Method
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Kargar Mohammad
Jahromi Fataneh Moein
Doosti Abbas
Handali Somayeh
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Abstract
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Objectives: The resistance of Streptococcus pneumoniae to the recently available antibiotic treatment has been a growing problem. The aim of the study was to determine the quinolone-resistant strains and detect the presence of mutations in the quinolone resistance-determining regions of the gyrA, parE, and parC genes.
Methods: In this study, for the first time in Iran, the polymerase chain reaction?restriction fragment length polymorphism (PCR-RFLP) method was used to investigate the presence of mutations at quinolone resistance-determining regions of topoisomerase IV and DNA gyrase on 82 S. pneumoniae strains, among them 45 clinical samples were from patients and 37 from healthy carriers (control group).
Results: In clinical samples, 34 (75.56%) strains contained mutations in the parC gene, 31 (68.89%) carried mutations in the gyrA gene, and 14 (31.11%) had parE gene mutations. Antibiotic susceptibility test was performed using the CLSI (Clinical and Laboratory Standards Institute) criteria on three different generations of quinolone family, with nalidixic acid (82.22%) showing the highest resistance and levofloxacin (42.22%) the least resistance.
Conclusion: Results indicated that there is a significant correlation between quinolone resistance development and mutations in the parE gene as well as in the parC and gyrA genes.
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KEYWORD
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gyrA, parC, parE, polymerase chain reaction?restriction fragment length polymorphism, quinolones, Streptococcus pneumoniae
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