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KMID : 1120220160070020083
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Osong Public Health and Research Perspectives
2016 Volume.7 No. 2 p.83 ~ p.89
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Comparison of Three Different Methods for Detection of IL28 rs12979860 Polymorphisms as a Predictor of Treatment Outcome in Patients with Hepatitis C Virus
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Fateh Abolfazl
Aghasadeghi Mohammadreza
Siadat Seyed D.
Vaziri Farzam
Sadeghi Farzin
Fateh Roohollah
Keyvani Hossein
Tasbiti Alireza H.
Yari Shamsi
Ataei-Pirkooh Angila
Monavari Seyed H.
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Abstract
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Objectives: This study aimed to evaluate the specificity, sensitivity, cost, and turn-around time of three methods of gene polymorphism analysis and to study the relationship between IL28B rs12979860 and SVR rate to pegIFN-α/RVB therapy among patients with chronic hepatitis C.
Methods: A total of 100 samples from chronic hepatitis C patients were analyzed in parallel using the three methods: direct sequencing, real-time polymerase chain reaction (PCR), amplification refractory mutation system (ARMS)-PCR.
Results: The different profiles for IL28B rs12979860 alleles (CC, CT, and TT) obtained with PCR-RFLP, ARMS-PCR, and direct sequencing were consistent among the three methods. Prevalence of rs12979860 genotypes CC, CT and TT in HCV genotype 1a was 10(19.6%), 35(68.6%), and six (11.8%), respectively, and in HCV genotype 31, it was 13(26.5%), 31(63.3%), and five (10.2%), respectively. No significant difference was seen between rs12979860 genotype and HCV genotype (p = 0.710).
Conclusion: Screening by ARMS ? PCR SNOP detection represents the most efficient and reliable method to determine HCV polymorphisms in routine clinical practice.
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KEYWORD
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amplification refractory mutation system (ARMS)-PCR, direct DNA sequencing, Hepatitis C, real-time polymerase chain reaction (PCR)
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