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KMID : 1120220200110010003
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Osong Public Health and Research Perspectives
2020 Volume.11 No. 1 p.3 ~ p.7
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Identification of Coronavirus Isolated from a Patient in Korea with COVID-19
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Kim Jeong-Min
Chung Yoon-Seok
Jo Hye-Jun
Lee Nam-Joo
Kim Mi-Seon
Woo Sang-Hee
Park Se-Hee
Kim Jee-Woong
Kim Heui-Man
Han Myung-Guk
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Abstract
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Objectives: Following reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients.
Methods: Upper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus.
Results: The virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells.
Conclusion: SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020.
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KEYWORD
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COVID-19, coronavirus, SARS-CoV-2, isolation, pneumonia
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