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KMID : 1134120120150040388
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Journal of Breast Cancer
2012 Volume.15 No. 4 p.388 ~ p.392
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Effect of bFGF on the MCF-7 Cell Cycle with CD44+/CD24-: Promoting the G0/G1→G2/S Transition
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Yang Zhen-lin
Cheng Kai
Han Zhao-dong
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Abstract
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Purpose: Few cells with stem cell characteristics possess capabilities of self-renewal and differentiation, which leads to high tumorigenesis and resistance to standard chemotherapeutic agents. These cells are mostly quiescent, and arrest occurs at the mitotic G0/G1 phase in mitosis. We explored the effects of basic fibroblast growth factor (bFGF) on the MCF-7 cell cycle with CD44+/CD24-.
Methods: Cancer-initiating cells were propagated as mammospheres. The CD44+/CD24- subpopulation was sorted by a fluorescence activating cell sorter-Vantage flow cytometer. A cell cycle analysis was performed with different bFGF concentrations.
Results: Differences in the CD44+/CD24- cell proliferation under different bFGF concentrations were observed (p=0.001). When the bFGF concentration was increased, the proportion of CD44+/CD24- at G0/G1 decreased (p=0.023).
Conclusion: We conclude that bFGF may sustain CD44+/CD24- cell proliferation and could promote cell progression through the G0/G1→G2/S phase transition.
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KEYWORD
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Breast neoplasms, Cell cycle, Fibroblast growth factors, Stem cells
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