KMID : 1134820080370030276
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Journal of the Korean Society of Food Science and Nutrition 2008 Volume.37 No. 3 p.276 ~ p.281
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Screening of Biological Activities of Extracts from
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Cho Young-Je
Ju In-Sik Chun Sung-Sook An Bong-Jeun Kim Jeung-Hoan
Kwon O-Jun
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Abstract
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Extracts from Rododendron mucronulatum Turcz. flowers were tested for antioxidant and their inhibitory
activities of ¥á-amylase, ¥á-glucosidase and angiotensin converting enzyme (ACE). Total contents of phenolics
were found as 30.6¡¾0.14 mg/g (60% EtOH extract) and 23.2¡¾0.21 mg/g (water extract). Electron donation
ability (EDA), ABTS [2,2azinobis(3-ethylbenzothiazoline-6-sulfonic acid)] radical decolorization, Antioxidant
protection factor (PF) and thiobarbituric acid reactive substance (TBARs) were measured for the antioxidative
activity of the extracts from Rododendron mucronulatum Turcz. flowers. The water extract were determined
as 97.5% at ethanol extract showed 83.2% and 60% EtOH extract were 89.7% in EDA. The water extract showed
higher antioxidant activity than 60% EtOH extract when evaluated by ABTS radical decolorization and
antioxidant PF. The TBARS of water extracts and 60% EtOH extracts were shown as 0.29¡¿102 ¥ìM and
0.28¡¿102 ¥ìM, respectively, and were lower than control. ACE inhibitory activity in water extract (67.6%
inhibition) was higher than that of 60% EtOH extract (46.7% inhibition) at 200 ¥ìg/mL. Water extracts had
higher inhibitory activities on ¥á-amylase and ¥á-glucosidase than 60% EtOH extracts. The result suggests
that the water extract from Rododendron mucronulatum Turcz. flowers will be useful as natural antioxidants
and functional foods.
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KEYWORD
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biological activities, Rododendron mucronulatum, antioxidant, ¥á-amylase, ¥á-glucosidase
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