KMID : 1134820180470090876
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Journal of the Korean Society of Food Science and Nutrition 2018 Volume.47 No. 9 p.876 ~ p.884
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Melatonin Content and Antioxidant Activity of Platycodon grandiflorum Seed Extract
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Woo Hye-Ryeon
Kim Yang-Ji Lee Yon Kim In-ho Kim Seok-Joong
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Abstract
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The melatonin content and antioxidant activity of Platycodon grandiflorum (PG) seed extract were measured and compared to those of PG sprout and root extracts. Among the solvents used to extract melatonin from seeds, 75% ethanol was better than methanol or 25, 50 or 100% ethanol (v/v), resulting in 146.8 ng melatonin per g extract by ELISA assay. The polyphenol content of seed extract prepared using 75% ethanol was 50.2 ¥ìg gallic acid equivalent (GAE)/g. This extract also exhibited dose-dependent antioxidant activity in various assays, showing 519.1 ¥ìmol Trolox equivalent (TE)/g in 2,2-diphenyl-1-picrylhydrazyl radical scavenging, 645.1 ¥ìmol TE/g in 2,2¡¯-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging, 471.1 ¥ìmol TE/g in ferric reducing antioxidant power, and 1,438.7 ¥ìmol TE/g in oxygen radical absorbance activity. Both the melatonin content and the antioxidant activity were highest in seed extract, followed by sprout and root extracts. Longer sprouting lowered the melatonin content and the antioxidant capacity. In a cellular antioxidant activity (CAA) test using 3T3-L1 cells, seed extract (40 ¥ìg/mL) showed 21.5 units CAA while neither sprout nor root extract exhibited any activity. These results suggest that PG seed is a novel functional foodstuff superior to its sprout and root in melatonin content and antioxidant activity.
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KEYWORD
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Platycodon grandiflorum seed, sprout, root, melatonin, antioxidant
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