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KMID : 1140120060110040297
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Cancer Prevention Research
2006 Volume.11 No. 4 p.297 ~ p.304
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A Study of Naphthalene Induced Gene Expression Changes in Mammalian 293T Cells Analyzed with DEG Assay
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An Jung-Min
Lee Na-Ri
Choi Soon-Young
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Abstract
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The naphthalene is a bicyclic aromatic hydrocarbon that is widely used domestically and commercially in moth repellents, lavatory scent discs and soil fumigants. The naphthalene causes lens opacification (cataracts), histological changes and carcinogenesis. However, there is little information regarding the mechanism of naphthalene toxicity. Previous studies in our laboratory have demonstrated that the naphthalene exposure decreased cell proliferation in diverse mammalian organ cells such as liver and kidney. And the naphthalene strongly induced apoptosis in 293T cells in a cell-specific manner by DNA fragmentation and caspase-3 activation. So, we studied to find the cellular factors that induce the apoptosis. Upon exposure to naphthalene for 24 hrs, we found that biomarker candidates of the naphthalene signal transduction by ACP-based PCR with the GeneFishingTM DEG assay. From this study, we found 16 different genes that were regulated as biochemical markers of toxicity. Ribosomal protein L12, large P0, translation initiation factor and nuclear protein STSG4523 were up-regulated. In contrast, trafficking protein particle complex4, leucyl tRNA synthetase, tubulin-specific chaperone D, cullin 1, mitochondrial cytochrome c oxidase subunit III, nuclear ribonucleoprotein H1, chondroitin sulfate proteoglycan 6 and speckle-type POZ protein were down-regulated. Also, we confirm the specifically down- or up-regulated 16 candidates mRNA levels by RT-PCR.
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KEYWORD
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Naphthalene, Viability, GeneFishing DEG assay, Biomarker
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