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KMID : 1140120080130030197
Cancer Prevention Research
2008 Volume.13 No. 3 p.197 ~ p.204
Protective Effects of Polygonatum odoratum Extracts on Amyloid ¥â-protein-induced Death in Neuronal Cells
Kim Eun-Sook

Choi Soo-Jin
Kang Ga-Hea
Oh Myung-Sok
Park Woo-Jin
Choi Young-Hwan
Lee Chul-Won
Lim Hak-Seob
Cho Yong-Kweon
Moon Ja-Young
Abstract
The amyloid ¥â-protein (A¥â) is the principal component of the senile plaques characteristic of Alzheimer¡¯s disease (AD) and elicits a toxic effect on neurons in vitro and in vivo. Many environmental factors including antioxidants and proteoglycans modify A¥â toxicity. In this study, we have investigated the protective effects of organic solvent-extracts of Polygonatum odoratum fractions pre-extracted with methanol on A¥â-induced oxidative cell death in cultured rat pheochromocytoma (PC12) cells. For this study, we used MTT reduction assay for detection of protective effects of organic solvent-extracts of Polygonatum odoratum fractions pre-extracted with methanol on A¥â25-35-induced cytotoxicity to PC12 cells. We also used cell-based ¥â-secretase assay system to investigate the inhibitory effect of organic solvent-extracts of Polygonatum odoratum on ¥â-secretase activity. We previously reported that methanol extracts of Polygonatum odoratum strongly attenuated cytotoxicity induced by the three A fragments (A¥â25-35, A¥â1-42 A¥â1-43) to both SK-N-MC and PC12 cells. In the present study, we found that the organic solvent extracts of Polygonatum odoratum fractions pre-extracted with methanol had the strong protective effects against A¥â25-35-induced cytotoxicity to PC12 cells and inhibitory potency to ¥â-secretase activity. These results suggest that organic solvent extracts of Polygonatum odoratum fractions pre-extracted with methanol may contain the protective component(s) against A¥â-induced cell death in PC12 cells as well
as inhibitory component(s) to ¥â-secretase activity. (Cancer Prev Res 13, 197-204, 2008)
KEYWORD
Amyloid ¥â-protein, Polygonatum odoratum, Liliaceae, PC12 cell, BACE, ¥â-secretase
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