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KMID : 1140320170010020088
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Precision and Future Medicine
2017 Volume.1 No. 2 p.88 ~ p.94
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Anti-fibrotic effect of bovine colostrum in carbon tetrachloride-induced hepatic fibrosis
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Sinn Dong-Hyun
Gwak Geum-Youn
Kwon Young-Ju
Paik Seung-Woon
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Abstract
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Purpose: To investigate the preventive effect of colostrum on hepatic fibrosis induced by carbon tetrachloride (CCl4) in mice.
Methods: Seven-week-old male Balb/C mice were treated with CCl4 with or without a diet of bovine colostrum (BC) for 2 weeks. Hepatic fibrosis was determined using Sirius red staining, α-smooth muscle actin (α-SMA) immunohistochemistry, immunoblot analysis, and quantitative polymerase chain reaction (PCR) for profibrogenic and proinflammatory genes. Hepatocellular injury was assessed through the measurement of serum alanine aminotransferase (ALT) levels.
Results: Compared to the CCl4 group, the BC4 group had a 55% reduction in fibrosis as demonstrated by Sirius red staining. The BC+CCl4 group also had a significant reduction in α-SMA expression according to immunohistochemistry and immunoblot. On quantitative PCR, the BC+CCl4 group showed significant 8.42-, 21.11-, 0.57-, and 2.90-fold reductions in the expression of Acta2, Col1α1, Tgfβ1, and Tnf-α, respectively, compared to the CCl4 group. The serum ALT level was significantly reduced in the BC+CCl4 group compared to the CCl4 group.
Conclusion: The present study demonstrated that BC ameliorated CCl4-induced hepatic fibrosis by inhibiting fibrogenesis-related gene expression and also decreased CCl4-induced hepatocyte injury. Therefore, BC might be therapeutically efficacious in preventing hepatic fibrosis in various liver diseases.
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KEYWORD
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Carbon tetrachloride, Colostrum, Fibrosis, Liver, Wounds and injuries
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