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KMID : 1140620180080030013
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CellMed
2018 Volume.8 No. 3 p.13 ~ p.13
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Effects of the Fraction of Sambucus Williamsii, NNMBS 246, on Osteoblastic Differentiation
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Kang Soon-Il
Park Jae-Suh
Kwon Il-Keun
Kim Eun-Cheol
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Abstract
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In the field of osteoporosis, there has been growing interest in anabolic agents that enhance bone formation. The purpose of this study was to examine the effects of NNMBS 246 osteoblastic differentiation with associated signaling pathways. NNMBS 246 markedly increased alkaline phosphatase (ALP) activity and calcium nodule formation. Stimulation with NNMBS 246 not only increased the differentiation markers (ALP, OPN, OCN) level and transcription markers (RUNX2, Osterix) mRNA expression but also upregulated the ECM molecules and OPG mRNA expression. Treatments of NNMBS 246 downregulated MMPs (MMP-1, MMP-2, MMP-9), but RANKL mRNA expression. Furthermore, NNMBS 246 activated osteoblastic differentiation markers and formed calcium nodules in human periodontal ligament cells (hPDLCs) and cementoblast cells. NNMBS 246 induced phosphorylation of MAPKs, Akt, nuclear p65 and IkB-α. BMP-2/Smad and β-catenin signaling pathways were activated by NNMBS 246. Sirtinol (SIRT1 inhibitor) inhibited NNMBS 246-induced osteoblastic differentiation markers mRNA expression. These results suggested that NNMBS 246 has the potential to enhance osteoblastogenesis probably through the activation of BMP/Smad and β-catenin signal pathways, and
SIRT1 plays as critical mediator in bone anabolic effect of NNMBS 246.
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KEYWORD
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NNMBS 246, Osteoblast, Differentiation, BMP Pathway, SIRT1 Pathway
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