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KMID : 1143620130170010080
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Korean Journal of Nuclear Medicine Technology
2013 Volume.17 No. 1 p.80 ~ p.84
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Simplification of One Step Immunotech AFP Test
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Moon Ki-Choon
Kwon Won-Hyun
Kim Jung-In
Lee In-Won
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Abstract
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Purpose: The Immunotech AFP is a widely used test in 14 hospitals(RIA QC establishments: 46) as a liver tumor marker. Normally, the test takes 45 minutes including complicated 2 step method. We evaluated the possibility of 1 step method and suitable test time.
Materials and Methods: Samples of 31 patients in SNUBH were used as subject. We evaluated the sensitivity, intra and inter assay precision, recovery rate, linearity between 1 step and 2 step method (45 min. and 60 min.).
Results: Both of 45 minutes and 60 minutes test showed 0.1 ng/mL sensitivity. In 45 minutes test, the intra assay coefficients of variation were 3.05%, 3.43%, 1.68%. Also, inter assay coefficients of variation were 3.91%, 2.38%, 0.82%. In 60 minutes test, the intra assay were 5.00%, 3.69%, 1.97%, inter assay were 3.14%, 3.71%, 1.85%.The correlation coefficient of each 2 step and 1 step (45 min.), 2 step and 1 step(60 min.), 1 step (45 min.) and 1 step (60 min.) were y=0.9293x+2.7356 R²=0.9999), y=0.9193x+4.1002 (R²=0.9993), y=0.9894x+1.3805 (R²=0.9997).
Conclusion: Compared 1step method to 2 step method, both method showed very reasonable precision, recovery rate and correlation coefficient. Also, 1 step method(45 min.) of correlation coefficient (R²) was 0.999. We suggest that 1 step method test can reduce the test time and is useful for AFP test.
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KEYWORD
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Precision, Recovery rate, Correlation coefficient
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