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KMID : 1148920090430040323
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Nuclear Medicine and Molecular Imaging
2009 Volume.43 No. 4 p.323 ~ p.329
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Comparison of Batch Assay and Random Assay Using Automatic Dispenser in Radioimmunoassay
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Moon Seung-Hwan
Lee Ho-Young
Shin Sun-Young
Min Kyung-Sun
Lee Hyun-Ju
Jang Su-Jin
Kang Ji-Yeon
Lee Dong-Soo
Chung June-Key
Lee Myung-Chul
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Abstract
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Purpose: Radioimmunoassay (RIA) was usually performed by the batch assay. To improve the efficiency of RIA without increase of the cost and time, random assay could be a choice. We investigated the possibility of the random assay using automatic dispenser by assessing the agreement between batch assay and random assay.
Materials and Methods: The experiments were performed with four items; Triiodothyronine (T3), free thyroxine (fT4), Prostate specific antigen (PSA), Carcinoembryonic antigen (CEA). In each item, the sera of twenty patients, the standard, and the control samples were used. The measurements were done 4 times with 3 hour time intervals by random assay and batch assay. The coefficient of variation (CV) of the standard samples and patients’ data in T3, fT4, PSA, and CEA were assessed. ICC (Intraclass correlation coefficient) and coefficient of correlation were measured to assessing the agreement between two methods.
Results: The CVs (%) of T3, fT4, PSA, and CEA measured by batch assay were 3.2±1.7%, 3.9±2.1%, 7.1±6.2%, 11.2±7.2%. The CVs by random assay were 2.1±1.7%, 4.8±3.1%, 3.6±4.8%, and 7.4±6.2%. The ICC between the batch assay and random assay were 0.9968 (T3), 0.9973 (fT4), 0.9996 (PSA), and 0.9901 (CEA). The coefficient of correlation between the batch assay and random assay were 0.9924(T3), 0.9974 (fT4), 0.9994 (PSA), and 0.9989 (CEA) (p<0.05).
Conclusion: The results of random assay showed strong agreement with the batch assay in a day. These results suggest that random assay using automatic dispenser could be used in radioimmunoassay.
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KEYWORD
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Radioimmunoassay, batch assay, random assay, standard curve, coefficient of variation, intraclass correlation coefficient
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