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KMID : 1148920100440030165
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Nuclear Medicine and Molecular Imaging
2010 Volume.44 No. 3 p.165 ~ p.169
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Early Metabolic Flare in Squamous Cell Carcinoma after Chemotherapy is a Marker of Treatment Sensitivity In Vitro
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Bjurberg Maria
Abedinpour Parisa
Brun Eva
Baldetorp Bo
Borgstrom Per
Wennerberg Johan
Kjellen Elisabeth
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Abstract
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Purpose: Early metabolic response with a decrease in glucose demand after cytotoxic treatment has been reported to precede tumor volume shrinkage. However, preclinical studies report of a very early rise in metabolism, a flare, following treatment. To elucidate these observations, we performed an experimental study on early metabolic response with sequential analysis of metabolic changes.
Methods: Three squamous cell carcinoma cell lines and one nontumorigenic cell line were exposed to cisplatin. The uptake of the fluorescent glucose analogue 2-NBDG was examined at days 1?6 using fluorescence microscopy. The relation between 2-NBDG-uptake and cell survival was evaluated.
Results: The tumor cells exhibited a high uptake of 2-NBDG, whereas the uptake in the nonmalignant cells was low. The more cisplatin sensitive cell lines exhibited a more pronounced metabolic flare than the less sensitive cell line.
Conclusion: A metabolic flare was a very early sign of treatment response and potentially it could be used as an early marker of treatment sensitivity.
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KEYWORD
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FDG-PET, NBDG, Flare, Fluorescence, Chemotherapy, Squamous cell carcinoma
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