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KMID : 1148920180520020144
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Nuclear Medicine and Molecular Imaging
2018 Volume.52 No. 2 p.144 ~ p.153
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123I?Labeled oxLDL Is Widely Distributed Throughout the Whole Body in Mice
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Nakano Atushi
Kawashima Hidekazu
Miyake Yoshinori
Zeniya Tsutomu
Yamamoto Akihide
Koshino Kazuhiro
Temma Takashi
Fukuda Tetsuya
Fujita Yoshiko
Kakino Akemi
Kanaya Shigehiko
Sawamura Tatsuya
Iida Hidehiro
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Abstract
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Purpose: Oxidized low-density lipoprotein (oxLDL) plays a key role in endothelial dysfunction, vascular inflammation, and atherogenesis. The aim of this study was to assess blood clearance and in vivo kinetics of radiolabeled oxLDL in mice.
Methods: We synthesized 123I?oxLDL by the iodine monochloride method, and performed an uptake study in CHO cells transfected with lectin-like oxLDL receptor-1 (LOX-1). In addition, we evaluated the consistency between the 123I?oxLDL autoradiogram and the fluorescence image of DiI-oxLDL after intravenous injection for both spleen and liver. Whole-body dynamic planar images were acquired 10 min post injection of 123I?oxLDL to generate regional time-activity curves (TACs) of the liver, heart, lungs, kidney, head, and abdomen. Regional radioactivity for those excised tissues as well as the bladder, stomach, gut, and thyroid were assessed using a gamma counter, yielding percent injected dose (%ID) and dose uptake ratio (DUR). The presence of 123I?oxLDL in serum was assessed by radio-HPLC.
Results: The cellular uptakes of 123I?oxLDL were identical to those of DiI-oxLDL, and autoradiograms and fluorescence images also exhibited consistent distributions. TACs after injection of 123I?oxLDL demonstrated extremely fast kinetics. The radioactivity uptake at 10 min post-injection was highest in the liver (40.8 ± 2.4% ID). Notably, radioactivity uptake was equivalent throughout the rest of the body (39.4 ± 2.7% ID). HPLC analysis revealed no remaining 123I?oxLDL or its metabolites in the blood.
Conclusion: 123I?OxLDL was widely distributed not only in the liver, but also throughout the whole body, providing insight into the pathophysiological effects of oxLDL.
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KEYWORD
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Oxidized low-density lipoprotein (oxLDL), Radiolabeled ligand, Dynamic planar imaging, Biodistribution
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