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KMID : 1160220000280020093
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Mycobiology
2000 Volume.28 No. 2 p.93 ~ p.96
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Genetic Relatedness of Korean Isolates of Pythium Using Restriction Fragment Length Polymorphism of PCR Amplified Ribosomal DNA and M-13 Marker
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Chee Hee-Youn
Kim Jin-Won
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Abstract
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Twenty-five isolates of Pythium species were identified and classified on the basis of RFLP(restriction fragment length polymorphism) of ITS(internal transcribed spacer) region in ribosomal DNA and M-13 PCR markers. Eight restriction endonucleases were used to investigate the genetic relatedness among isolates. P. graminicola and P arrhenomanes as well as P. aphanidermatum and P. delience produced identical band patterns with all restriction endonucleases used and M-13 markers. P. myriotylum and P. catenulatum also formed tight cluster on the basis of RFLP of ribosomal DNA but produced distinct band pattern with M-13 PCR markers. No intraspecific variations were observed with RFLP of ITS region in ribosomal DNA. Molecular analysis based on M-13 marker demonstrated that each species produced distinct band patterns. Intraspecific variation of P. ultimum and P. torulosum was observed with M-13 markers.
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KEYWORD
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Pythium, Genetic relatedness, Restriction fragment length polymorphism, Internal transcribed spacer, ribosomal DNA, M-13 marker
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