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KMID : 1160220000280040171
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Mycobiology
2000 Volume.28 No. 4 p.171 ~ p.176
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Genomic Organization of ancop Gene for ${\alpha}-COP$ 수식 이미지 Homolog from Aspergillus nidulans
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Lee Hwan-Hee
Chae Shun-Kee
Kim Jeong-Yoon
Maeng Pil-Jae
Park Hee-Moon
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Abstract
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We have cloned a α-COP homolog, ancop, from Aspergillus nidulans by colony hybridization of chromosome specific library using α-COP homologous fragment as a probe. The probe DNA was amplified with degenerated primers designed by comparison of conserved region of the amino acid sequences of Saccharomyces cerevisiae α-COP, Homo sapiens HEP-COP, and Drosophila melanogaster α-COP. Full length cDNA clone was also amplified by RT-PCR. Comparison of genomic DNA sequence with cDNA sequence obtained by RT-PCR revealed 7 introns. Amino acid sequence similarity search of the anCop with other α-COP gave an overall identity of 52% with S. cerevisiae, 47% with human and bovine, 45% with Drosophila and Arabidopsis. In upstream region from the transcription start site, a putative TATA and CAAT motif were also identified.
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KEYWORD
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Aspergillus nidulans, α-COP, Genomic structure, Sequence homology
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